Header

Search
Animal Welfare and 3Rs

Publications Paulin Jirkof

Original Research Articles

  1. Thallmair M, Jirkof P. A scoping review on reporting of methods in DSS colitis mouse models. Laboratory Animals. 2025;0(0). doi:10.1177/00236772251331677
  2. Steiner S, Krzyzaniak O, Nilsson FAM, et al. Sex and strain differences in the micropipette-guided drug administration (MDA) method in mice. Laboratory Animals. 2025;0(0). doi:10.1177/00236772251320843
  3. Krzyzaniak, O., Steiner, S., Nilsson, F. A., Dietrich, M., Kämpfen, L., Johansen, P., ... & Meyer, U. (2025). Applicability of the micropipette-guided drug administration (MDA) method for assessing reward-related behaviors in mice. Physiology & Behavior, 114967.

  4. Munk, A., Philippi, V., Buchecker, V., Bankstahl, M., Glasenapp, A., Blutke, A., Jirkof, P. ... & Potschka, H. (2024). Refining pain management in mice by comparing multimodal analgesia and NSAID monotherapy for neurosurgical procedures. Scientific Reports, 14(1), 18691.

  5. Wolter, A., Jirkof, P., Thöne-Reineke, C., Rapp, A. E., & Lang, A. (2024). Evaluating rearing behaviour as a model-specific pain indicator in mouse osteotomy models. Laboratory Animals, 58(1), 9-21.
  6. King, H., Reiber, M., Philippi, V., Stirling, H., Aulehner, K., Bankstahl, M., ..Jirkof, P.. & Potschka, H. Anesthesia and analgesia for experimental craniotomy in mice and rats: A systematic scoping review comparing the years 2009 and 2019. Frontiers in Neuroscience, 17, 595.10.3389/fnins.2023.1143109 
  7. Spalinger M., Schwarzfischer M., Niechcial A., Atrott K., Laimbacher A., Jirkof P., & Scharl M. (2023). Evaluation of the effect of tramadol, paracetamol and metamizole on the severity of experimental colitis. Laboratory Animals, 00236772231163957.
  8. Wolter A., Bucher C. H., Kurmies S., Schreiner V., Konietschke F., Hohlbaum K.,... Jirkof P & Lang, A. (2023). A buprenorphine depot formulation provides effective sustained post-surgical analgesia for 72 h in mouse femoral fracture models. Scientific Reports, 13(1), 3824.
  9. Talbot SR, Struve B, Wassermann L, Heider M, Weegh N, Knape T, Hofmann MCJ, von Knethe A, Jirkof P, Häger C, Bleich A (2022). RELSA—A multidimensional procedure for the comparative assessment of well-being and the quantitative determination of severity in experimental procedures. Frontiers in Veterinary Science, 9.
  10. Aulehner K, Leenaars C, Buchecker V, Stirling H, Schönhoff K, King H, Häger C, Koska I, Jirkof P, Bleich A, Bankstahl M, Potschka H (2022) Grimace scale, burrowing and nest building for the assessment of postsurgical pain in mice and rats - a systematic review. Frontiers in Veterinary Science, 9.
  11. Gkrouzoudi, A., Tsingotjidou, A., & Jirkof, P. (2022). A Systematic Review on Reporting of Refinements Measures in Mouse ECG Telemetry Implantation Surgery. Laboratory Animals, 00236772221115492.
  12. Tappe-Theodor A, Pitzer C, Lewejohann L, Jirkof P, Siegeler K, Segelcke A, Drude N, Pradier B, Pogatzki-Zahn EM, Hollinderbäumer B, Segelcke D (2022) The "WWHow" concept for prospective categorization of post-operative severity assessment in Rodents. Frontiers in Veterinary Science, 9.
  13. Gkrouzoudi A., Tsingotjidou A., & Jirkof P (2021). A Systematic Review on the Reporting Quality in Mouse Telemetry Implantation Surgery Using Electrocardiogram Recording Devices. Physiology & Behavior, 113645.
  14. Durst M, Reding Graf T, Graf R, Kron M, Arras M, Zechner D, Palme R, Talbot S, Jirkof P (2021) Analysis of pain and analgesia protocols in acute Cerulein-induced pancreatitis in male C57BL/6 mice. Frontiers in Physiology. https://doi.org/10.3389/fphys.2021.744638
  15. Wolter A, Rapp AE, Durst M, Hildebrand L, Löhning M, Buttgereit F, Schmidt-Bleek K, Jirkof P, Lang A (2021) Systematic review on the reporting accuracy of experimental details in publications using mouse femoral fracture models. Bone, 116088.
  16. Schreiner V, Detampel P, Jirkof P, Puchkov M, Huwyler J (2021) Buprenorphine Loaded PLGA Microparticles: Characterization of a Sustained-Release Formulation. Journal of Drug Delivery Science and Technology. 63. https://doi.org/10.1016/j.jddst.2021.102558
  17. Durst M, Arras M, Palme R, Talbot S, Jirkof P (2021) Lidocaine and Bupivacaine as part of multimodal pain management in a C57BL/6J laparotomy mouse model. Scientific Reports. 11.1 (2021): 1-17.
  18. van der Mierden S, Leenaars CHC, Boyle EC, Ripoli FL, Gass P, Durst M, Goerlich-Jansson VC, Jirkof P, Keubler LM, Talbot SR, Habedank A, Lewejohann L, Tolba RH, Bleich A (2020) Measuring endogenous corticosterone in laboratory mice-a mapping review, meta-analysis, and open source database. ALTEX-Alternatives to animal experimentation.
  19. Schreiner V, Durst M, Arras M, Detampel P, Jirkof P, Huwyler J (2020) Design and In Vivo Evaluation of a Microparticulate Depot Formulation of Buprenorphine for Veterinary Use. Scientific Reports.
  20. Jirkof P, Bratcher, N., Medina, L. et al. (2020) The effect of group size, age and handling frequency on inter-male aggression in CD 1 mice. Scientific Reports. 10, 2253. https://doi.org/10.1038/s41598-020-59012-4
  21. Jirkof P, Rudeck J, Lewejohann L (2019) Assessing Affective State in Laboratory Rodents to Promote Animal Welfare—What Is the Progress in Applied Refinement Research? Animals, 9(12), 1026; https://doi.org/10.3390/ani9121026
  22. Jirkof P, Durst M, Thöne-Reinecke C, Palme R, Lang A (2019) Administration of Tramadol or Buprenorphine via the drinking water for post-operative analgesia in a mouse-osteotomy model. Scientific Reports 9, 10749 doi:10.1038/s41598-019-47186-5
  23. Jirkof P., Abdelrahman A, Bleich A, Durst M, Keubler L, Potschka H, Struve B, Talbot S, Vollmar B, Zechner D, Häger C (2019) A safe bet? Inter-laboratory variability in behavior-based severity assessment. Laboratory Animals. https://doi.org/10.1177/0023677219881481
  24. Ernst L, Kopaczka M, Schulz M, Talbot S, Struve B, Häger C, Bleich A, Durst M, Jirkof P, Arras M, v. Dijk R, Miljanovic N, Potschka H, Merhof D, Tolba R (2019) Semi-automated generation of pictures for the Mouse Grimace Scale: a multilab analysis (Part II). Laboratory Animals. https://doi.org/10.1177/0023677219881664
  25. Leenaars CHC, van der Mierden S, Durst  M, Goerlich-Jansson VC, Lüder Ripoli F, Keubler LM, Talbot SR, Boyle E, Habedank A, Jirkof P, Lewejohann L, Gass P, Tolba R, Bleich A (2019). Measurement of corticosterone in mice: a protocol for a mapping review. Laboratory Animals. https://doi.org/10.1177/0023677219868499
  26. Jirkof P, Schmutz J (2019) Social and organizational factors affecting biosafety compliance in animal facilities: An integrative analysis of safety rules within the system. Safety Science. 18:538-550. doi.org/10.1016/j.ssci.2019.05.053
  27. Evangelista-Vaz E, Bergadano A, Arras M, Jirkof P (2018) Evaluation of the analgesic efficacy of a 25 mg/kg Tramadol s.c. injection-oral combination in a surgical model in C57BL/6J mice. Journal of the American Association for Laboratory Animal Science. 57:4. DOI: 10.30802/AALAS-JAALAS-17-000118
  28. Jirkof P, Arras M, Cesarovic, N (2018) A Tramadol:Paracetamol combination for post-surgical pain treatment of mice via the drinking water. Applied Animal Behaviour Science. 189: 95-100. doi.org/10.1016/j.applanim.2017.09.021
  29. Lipiski M, Arras M, Jirkof P, Cesorovic N (2017) Premedication with Fentanyl-Midazolam improves Sevoflurane Anesthesia for Surgical Intervention in Laboratory Mice. Experimental Biology and Medicine. 242: 12, 1287-1298, doi. 10.1177/1535370217707730
  30. Jeger V, Arrigo M, Hildenbrand F, Müller D, Jirkof P, Hauffe T, Seifert B, Arras M, Spahn DR, Bettex D, Rudiger A (2017) Improving animal welfare using continuous nalbuphine infusion in a long-term rat model of sepsis. Intensive Care Medicine Experimental. 5: 23, doi.org/10.1186/s40635-017-0137-2
  31. Fleischmann T, Sauer M, Rülicke T, Arras M, Jirkof P (2016) Voluntary intake of paracetamol-enriched drinking water and its influence on the success of embryo transfer in mice. Research in Veterinary Science. 111: 85–92, doi.org/10.1016/j.rvsc.2016.12.005
  32. Sauer M, Fleischmann T, Lipiski M, Arras M, Jirkof P (2016) Buprenorphine for pain relief in mice: administration via the drinking water. Applied Animal Behaviour Science. 185: 103–112, doi.org/10.1016/j.applanim.2016.09.009
  33. Fleischmann T, Henke J, Jirkof P, Arras M, Cesarovic N (2016) Injection anaesthesia with fentanyl-midazolam-medetomidine in adult female mice: importance of antagonisation and peri-operative care. Laboratory Animals. 50 (4): 264-274, doi.org/10.1016/j.applanim.2016.09.009
  34. Husmann K, Arlt M, Jirkof P, Arras M, Born W, Fuchs B (2015) Primary tumour growth in an orthotopic osteosarcoma mouse model is not influenced by analgesic treatment with buprenorphine and meloxicam. Laboratory Animals. 49(4):284-93, doi: 10.1177/0023677215570989
  35. Jirkof P, Tourvielle A, Cinelli P, Arras M (2014) Buprenorphine for pain relief in mice: repeated injections vs sustained release depot formulation. Laboratory Animals. 49(3):177-87, doi: 10.1177/0023677214562849
  36. Schuler B, Arras M, Gianella M, Vogel O, Jirkof P, Cesarovic N, Klohs J, Jakob P, Baum O, Rieger G, Gubser M, Hoppeler H, Samillan-Soto V, Gassmann M, Fischer J, Born W, Vogel J (2014) Endogenous alpha calcitonin gene-related peptide (αCGRP) promotes physiological, exercise-induced cardiac hypertrophy. Acta Physiologica. 211(1):107-21, doi: 10.1111/apha.12244
  37. Wueest S, Item F, Boyle C, Jirkof P, Cesarovic N, Ellingsgaard H, Arras M, Donath M, Lutz T, Schoenle E, Konrad D (2014) Interleukin-6 as a mediator of early metabolic adaptation to fasting. American Journal of Physiology. 306(11): R861-7, doi: 10.1152/ajpregu.00533.2013
  38. Cesarovic N, Arras M, Jirkof P (2014) Impact of inhalation anaesthesia, surgery and analgesic treatment on home cage behaviour in laboratory mice. Applied Animal Behaviour Science. 157: 137–145, doi: 10.1016/j.applanim.2014.04.010
  39. Jirkof P, Fleischmann T, Cesarovic N, Rettich A, Vogel J, Arras M (2013) Assessment of post-surgical distress and pain in laboratory mice by nest complexity scoring. Laboratory Animals. 47:153-61, doi:10.1177/0023677213475603
  40. Jirkof P, Leucht K, Cesarovic N, Krebs M, Nicholls F, Rogler G, Hausmann M, Arras M (2013) Burrowing is a sensitive behavioral assay for monitoring general wellbeing during dextran sulfate sodium colitis in laboratory mice. Laboratory Animals. 47(4):274-83, doi:10.1177/0023677213493409
  41. Jirkof P, Cesarovic N, Rettich A, Arras M (2013) Housing of female mice in a new environment and its influence on post-surgical behaviour and recovery. Applied Animal Behaviour Science. 148, 209–217, doi: 10.1016/j.applanim.2013.08.006
  42. Cesarovic N, Jirkof P, Rettich A, Nicholls F, Arras M (2012) Combining sevoflurane anesthesia with injection of fentanyl-midazolam or S-ketamine in laboratory mice. Journal of the American Association for Laboratory Animal Science. 51(2):209–18
  43. Arras M, Glauser D, Jirkof P, Rettich A, Schade B, Cinelli P, Pinschewer D, Ackermann M (2012) Multiparameter telemetry as a sensitive screening method to detect vaccine reactogenicity in mice. PLoS ONE. 7(1): e29726, doi: 10.1371/journal.pone.0029726
  44. Jirkof P, Cesarovic N, Fleischmann T, Rettich A, Arras M (2012) Individual housing of female mice: influence on postsurgical behaviour and recovery. Laboratory Animals. 46(4):325-34, doi: 10.1258/la.2012.012027
  45. Cesarovic N, Jirkof P, Rettich A, Arras M (2011) Implantation of radiotelemetry transmitters yielding data on ECG, heart rate, core body temperature and activity in free-moving laboratory mice. Journal of Visualized Experiments. 57: e3260, doi: 10.3791/3260
  46. Cesarovic N, Nicholls F, Rettich A, Kronen P, Hässig M, Jirkof P, Arras M (2010) Isoflurane and sevoflurane provide equally effective anaesthesia in laboratory mice. Laboratory Animals. 44 (4):329-36
  47. Jirkof P, Cesarovic N, Rettich A, Nicholls F, Seifert B, Arras M (2010) Burrowing behavior as an indicator of post-laparotomy pain in mice. Frontiers in Behavioral Neuroscience. 4:165, doi:10.3389/fnbeh.2010.00165

Invited Review Articles

  1. Jirkof P (2017) Side effects of pain and analgesia in animal experimentation. Lab Anim (NY). 46(4):123-128, doi: 10.1038/laban.1216

  1. Jirkof P (2015) Effects of experimental housing conditions on recovery of laboratory mice. Lab Animal (NY). 44, 65 – 70, doi:10.1038/laban.662

  1. Jirkof P (2014) Monitoring Burrowing and Nest Building Behavior as Species-Typical Indicators of Animal Well-being in Mice. Journal of Neuroscience Methods. 234:139-46, doi: 10.1016/j.jneumeth.2014.02.001

Other Articles

  1. Tremoleda JL, Cederroth C, Jirkof P. Engaging on discussions about the use and the replacement of animals in research with young minds. Laboratory Animals. 2023;0(0). doi:10.1177/00236772221147134Jirkof P (2022) Refining Research to Improve the Lives of Laboratory Mice. Front. Young Minds. 10:954413. doi: 10.3389/frym.2022.954413
  2. Fachinformation: Tiergerechte Haltung von Wüstenrennmäusen im tierexperimentellen Bereich, Ausschuss Tiergerechte Haltung, GV SOLAS, 2021
  3. Fachinformation: Empfehlung zur Schmerztherapie bei Versuchstieren. Ausschuss Anesthesie und Analgesie, GV SOLAS , 2020
  4. Fachinformation: Tiergerechte Haltung von Laborkaninchen, Ausschuss Tiergerechte Haltung, GV SOLAS, 2020
  5. Lewejohann, L., Schwabe, K., Häger, C., & Jirkof, P. (2020). Impulse for animal welfare outside the experiment. Laboratory Animals.
  6. Jirkof P, Seebeck P (2019) Vital signs without handling or animal prep. Lab Anim. DOI: 10.1038/s41684-019-0290-1
  7. Riederer B, Bomzon A, Jirkof P (2017) Scientific Writing in the Laboratory Animal Sciences. Letter from the editors. Laboratory Animals. 51(3), 315-320
  8. Golledge H, Jirkof P (2016) Editorial: Score sheets and analgesia. Laboratory Animals. 50(6):411-413
  9. Fachinformation: Tiergerechte Haltung von Labormäusen, Ausschuss Tiergerechte Haltung, GV SOLAS, 2014
  10. Stellungnahme: Einzelhaltung von Mäusen zu Versuchszwecken, Ausschuss Tiergerechte Haltung, GV SOLAS, 2013

Book chapter

  1. Jirkof P, Potschka H, Taylor D (2023) Optimizing anesthesia and analgesia for specific research projects. In: Anesthesia and Analgesia in Laboratory Animals, 3rd Edition.

  2. Jirkof P, Lofgren J (2023) Anesthesia and analgesia in laboratory rodents. In: Anesthesia and Analgesia in Laboratory Animals, 3rd Edition.

  3. Jirkof P, Potschka H. Effects of untreated pain, anesthesia and analgesia in animal experimentation. In: Experimental Design and Reproducibility in Preclinical Animal Studies. ESLAV, 2021
  4. Baumans V, Brain P, Jirkof P, Hendriksen C. Chapter 14 Recognition of pain and distress. In Handbook on Laboratory Animal Science edited by van Zutphen, Baumans and Beynen. 2021