Replacement: Alternatives to Animal Experimentation

Websites with 3R resources

Overview 3R website: Replacement

UC Davis Database


Database for alternative methods to animal experiments.

AnimAlt-ZEBET Database


Resources for Alternatives to the Use of Live Vertebrates in Biomedical Research and Testing.
ALTBIB Database


The Biomedical Research for the 21st Century (BioMed21) Collaboration

This collaboration brings together scientists and institutions from across Europe, Asia and the Americas who share a vision of a new, human-focused paradigm in health research.


DC 3Rs - Inventory of 3Rs Knowledge Sources

A study carried out by the European Commission’s Joint Research Centre compiled knowledge sources (websites, publications, databases, organisations, events, expert groups, etc.) relevant to the 3Rs. This study, is now available.

To make the most of this knowledge base, DC has developed an online application to allow sharing. Theapp allows the user to consult 3Rs knowledge sources listed in the JRC study.

DC App

EURL ECVAM Search Guide

The EURL ECVAM Search Guide provides search principles and procedures, suggested search terms and user guidance together with an inventory of relevant information resources to support untrained database users in finding high quality information on relevant alternative strategies and methods. 

It can be downloaded (pdf) or requested in paper copy (for free) from the EU Bookshop.

EURL ECVAM DataBase on ALternative Methods (DB-ALM) / EU Scieence Hub

Collection of data-sets providing factual information (not only bibliographic references) presented as evaluated (and therefore ready-to-use) data sheets.

EURL ECVAM Review of non-animal models in biomedical research - Immuno-oncology

JRC Data catalogue on non-animal methods in neurodegenerative diseases research

European Organ-on-Chip Society (EUROoCS)

is an independent, not-for-profit organization established to encourage and develop Organ-on-Chip research, and to provide opportunities to share and advance knowledge and expertise in the field towards better health for all. .


FCS-free Database

Fetal Calf Serum (FCS, also known as Fetal Bovine Serum, or FBS) is a common supplement of animal cell culture media. However, moral and scientific concerns demonstrate the urgency to switch to an FCS-free medium. The FCS-free Database, part of the 3Rs Database Programme, provides an overview of FCS-free media for cell-culture. A forum function for each record allows researchers to discuss the applicability of each product. The database is offered by the 3Rs-Centre ULS in collaboration with Animal Free Research UK.

see also SEFREC (Serum free cell lines)

ISSG search filter

This Search Filter Resource is a collaborative venture to identify, assess and test search filters designed to retrieve research by study design or focus. The search filters resource aims to provide easy access to published and unpublished search filters. It also provides information and guidance on how to critically appraise search filters, study design filters in progress and information on the development and use of search filters. Inclusion of a search filter is not an endorsement of its validity or a recommendation.
ISSG filters

Microphysiology Systems Database

The MPS-Db aggregates MPS data with linked preclinical and clinical databases for analysis in the context of human exposure


The NAT database (Non-Animal Technologies)

contains information on modern non-animal technologies from various areas of biomedicine and life sciences, based on scientific studies and publications.


Recombinant Antibodies Database (Biocompare)

Recombinant Antibodies are produced without the use of laboratory animals. Researchers can use the Biocompare database to search for them. Biocompare is a resource for up-to-date product information, product reviews, and new technologies for life scientists.



New Approach Methodologies (NAMs) database: In vitro, ex vivo, in silico and in chemico alterntaives to animal experiments.


VirtualToxLab - Biograf 3R

The VirtualToxLab is an in silico tool for predicting the toxic potential (endocrine and metabolic disruption, some aspects of carcinogenicity and cardiotoxicity) of drugs, chemicals and natural products.


Webinars on 3Rs topics.